EFFECT OF Anti-miRNA 144 AND Anti-miRNA 150 ON THE EXPRESSION OF α GLOBIN CHAINS IN PBMC

The excess of unbound α globin chains are the basic pathophysiology cause clinical symptoms in major β thalassemia. Recently there many alternative therapies by increasing γ to reduced effects chains. Alternative decreasing have not been much noticed. expression involves complex regulatory involving transcription factors and miRNAs. It GATA-1, KLFD MYB . also miRNA-144 miRNA-150. role miRNA-150 reduce thalassemia patients is an therapy. miRNA150 activity need be known using anti-miRNA 144 anti- miRNA 150. Analyzed effect 150 on This study was experimental PBMC a patients. divided into four groups that were transfected, transfected 144, qPCR examinations find out Western blot examination There significantly lower who had than those transfected. In line with result which 0.17 times control group. 0.30 evidenced decreased area 10-25 KDa band. Based this study, administration or capable